Catalog#/Size：AC52350/100 µg.

Source：Escherichia coli.

Molecular Weight：Approximately 15.5 k Da, a single non-glycosylated polypeptide chain containing 134 amino acid.

Description：Accession #P60568.1, Ala21-Thr153（ C125S）, with an N-terminal Met.

SDS-PAGE：15.5k Da, reducing conditions.

Purity：>95%, as determined by SDS-PAGE, under reducing non-reducing conditions, visualized by coomassie staining.

Endotoxin：Less than 0.1 EU/ µg of rHuIL-2 as determined by kinetic Limulus Amoebocyte Lysate (LAL) assay.

Biological Activity：Recombinant human IL-2 bioactivity is measured in a cell proliferation assay using CTLL-2 mouse cytotoxic T cells, the EC50 for this effect is 2.492 to 3.037 ng/mL.

Physical Appearance： Sterile Filtered White lyophilized (freeze-dried) powder

Formulation：Lyophilized from a 0.2 µm filtered concentrated solution in 20 mM PB pH 3.5 with 10% trehalose anhydrous.  
Reconstitution：We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute  to  a  concentration  of  0.1-1.0 mg/mL  in  sterile  distilled   H₂O.  Stock  solutions  should   beapportioned  into working  aliquots  and  stored  at-20℃ to-70℃ .  Further  dilutions  should  be  made  in appropriate buffered solutions. Do not reconstitute in cell culture media directly.

Shipping：The product is shipped at 2℃ to 8℃ . Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage：Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

         A minimum of 12 months from date of shipping when stored at -20℃ to-70℃ as supplied. 

         4 weeks at 2℃ to 8℃ under sterile conditions after reconstitution.

         4 months at -20℃ to-70℃ under sterile conditions after reconstitution.

Usage：Acnovia rHuIL-2 product can be used for a variety of ex vivo cell culture applications such as research or further manufacturing.

Quality statement：No animal- or human-derived materials were used for the manufacture of this product, unless otherwise stated in the respective Certificate of Origin.

IL-2, also named T-cell growth factor, was first discovered in 1976 and was characterized as a soluble factor with the unique ability to promote clonal expansion of many hematopoietic lines including regulatory T cells (Tregs) in vitro. Human IL-2 has 133 amino acids and in mature form it is a glycosylated globular protein of 15.5 k Da four-bundle,  α  -helical protein member of the common cytokine receptor  γ-chain family of cytokines. IL-2 is predominantly produced by activated CD4⁺ T cells and, to a lesser extent by activated CD8⁺ T cells, activated dendritic cells, natural killer (NK) cells, NKT cells, as well as B cells.

1. Liao W, et al. 2013. Immunity. 38:13-25.

2. Sakaguchi S, et al. 2008. Cell. 133:775-87.

3. Kryczek, I. et al. 2007. J. Immunol. 178:6730.

4. Cote-Sierra J, et al. 2004. Proc Natl Acad Sci USA. 101:3880-5.