Catalog#/Size：AC52381/100 µg.

Source: Escherichia coli.

Molecular Weight: Approximately 16.9kDa, a single non-glycosylated polypeptide chain containing 143 amino acids.

Description：Accession # CAA31639, Gln24-Gln166, with an N terminal Met.

SDS-PAGE: 16.9 kDa, under reducing conditions.

Purity: > 95 %, as determined by SDS-PAGE, under reducing non-reducing conditions, visualized by coomassie staining.

Endotoxin: Less than 0.01 EU/µg of rHuIFN-γ as determined by kinetic Limulus Amoebocyte Lysate (LAL) assay.

Biological Activity: Recombinant human IFN-γ bioactivity is measured by inhibition of the proliferation of HT-29 cells, the EC50 for this effect is 8.342-14.89 ng/mL.

Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation: Lyophilized from a 0.2 μm filtered solution in Sodium Succinate, Mannitol and Tween 80.

Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute to a concentration of 0.1-1.0 mg/mL in sterile distilled H2O. Stock solutions should be apportioned into working aliquots and stored at-20 ℃ to -70 ℃. Further dilutions should be made in appropriate buffered solutions. Do not reconstitute in cell culture media directly.

Shipping: The product is shipped at 2 °C to 8 ° C. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

               A minimum of 12 months from date of shipping when stored at -20℃ to -70℃ as supplied. 

               4 weeks at 2 ℃ to 8 ℃ under sterile conditions after reconstitution. 

               4 months at -20 ℃ to -70 ℃ under sterile conditions after reconstitution

Usage: Acnovia rHu IFN-γ product can be used for a variety of ex vivo cell culture applications such as research or further manufacturing.

Quality statement: No animal- or human-derived materials were used for the manufacture of this product, unless otherwise stated in the respective Certificate of Origin.

IFN-γ is a homodimer glycoprotein formed by antiparallel association of two 21–24-kD subunits not covalently linked. Each subunit has six alpha helices held together by short nonhelical sequences that is primarily produced by natural killer (NK) cells, T cells, and NK T cells. However, IFN-γ is also secreted by other cells such as B cells, macrophages, and dendritic cells (DCs). IFN-γ plays essential and unique immune and biological roles beyond its well-known antiviral function. As the sole type II class IFN, the biologically active form of IFN-γ is a dimer. A primary role for IFN-γ is the activation of macrophages to increase phagocytosis, tumoricidal properties, and intracellular killing of pathogens, particularly bacteria and fungi. IFN-γ induces macrophage production of a variety of inflammatory mediators and reactive oxygen and nitrogen intermediates.

1.Kak, Gunjan, Raza, Mohsin and Tiwari, Brijendra K. "Interferon-gamma (IFN-γ): Exploring its implications in infectious diseases" Biomolecular Concepts, vol. 9, no. 1, 2018, pp. 64-79.

2.Heekyong Bae, Alec T. Barlow, Howard Young, Julio C. Valencia, Interferon γ: An Overview of Its Functions in Health and Disease,Editor(s): Michael J.H. Ratcliffe, Encyclopedia of Immunobiology, Academic Press, 2016, Pages 494-500.